PCR And Sequencing Technique Detection Of Gardnerella Spp Associated With Urinary Tract Vaginosis

Abstract

Introduction: Gardnerella is a bacteria originally described in bacterial vaginosis, which can colonize the urinary tract and is often associated with vaginosis when present as a single-species flora. Commonly found in vaginal tracts, and it is also associated genital tract problems.

Methods: The current study was to identify the genetic variation of 16SrRNA gene in Gardnerella  spp. which isolated from urine samples. In this study, 100 samples of urine from of women suffering from Urinary tract problems collected between August to September 2023 in  Thi-Qar province of Iraq.

Results: The PCR technique results noted that (90%) of urine samples had Gardnerella sp. isolates by 16SrRNA gene. The bacterial samples recorded in NCBI (LC774556, LC774557, LC774558, LC774559, LC774560 LC7745561, LC774562 and LC774563) a 278bp genetic fragment that partially encompassed a coding region of the 16SrRNA gene was enlarged in this study. The phylogenetic tree was built in order to evaluate the precise phylogenetic distances, in addition to other related bacterial genomes. The present results  exhibited that Gardnerella spp. sequences showed around 99% homology. Genetic variations of 16SrRNA gene were identified, the major variations were variably distributed in the (LC774556, LC774557) of the studied samples; while the variation in (G176A) was identified only in LC774556 sample. Also, the deletion mutation (A116 del) was noticed in all studied samples, while (T129 del) was showed in (LC774556, LC774558, LC774560) samples.

Conclusion: The infection with Gardnerella vaginalis. may be related with Bacterial vaginosis tract; Also, the DNA sequencing used to detect the genetic variation of Gardnerella vaginalis.