Study the Effect of Mango Peel Extract on Pathogenic Bacteria Isolated From Urinary Tract Infection

Abstract

Objective:  Urinary tract infection is an infection that affects any part of the urinary system. The urinary system includes the kidneys, ureters, bladder, and urethra. Most infections affect the lower part of the system, the bladder and urethra. The infection may reach the kidneys and develop into kidney failure. This study aimed to find a new natural extract against the germs that cause the disease to help reduce the large number of patients suffering from the disease. Methods: This study included plant extraction for mango peels by used ethanol 70% in soxhlet apparatus. Urine samples were collected from dogs, and samples containing inflammatory cells were isolated and cultured. The cultured bacteria were identified by used morphology of colony, Gram stain and biochemical tests. Biofilm made by used microliter plate method, activity of mango peels extract (MPE) was measured by well agar diffusion method, the concentration of minimal inhibitory (MIC), and the concentration of minimal bactericide (MBC) was measured by broth dilution method, Statistical analysis was used to determine the effectiveness of the extract. Results: Diagnosis for isolated pathogenic bacteria of urine samples which contained inflammatory cells staph aureus, E.coli,  pseudomonas.  The best Biofilm formation of staph aureus (91.7)%  followed by E.coli ( 83.4),  pseudomonas (16.7). The highest effectiveness of mango peels extract activity was recorded against staph aureus (22-26) mm zone of inhibition and (17-19)mm of E.coli ciprofloxacin gave (22-25)mm zone of inhibition of S.aureus and (18-22)mm E.coli . The concentration of minimal inhibitory (2mg/ml) of staph aureus and (4mg/ml)  E.coli  The concentration of minimal bactericide (4mg/ml)  of staph aureus  and (8mg/ml)   E.coli. The Statistical analysis of inhibition halos of staph aureus (24  ± 0.6  )  highest than  E.coli (18 ± 0.2) of MPE and the Statistical analysis of inhibition halos of staph aureus(23± 0.2) and(20± 0.6 )   E.coli  of ciprofloxacin. Conclusion: Mango peels extract have antibacterial effect against both gram positive and gram negative pathogenic bacteria isolated from UTI. Use of extract is help in treating patients with urinary tract infection, MPE contributing and limiting the progression of the condition.