Synergistic Effect of Aloe Vera L. Extract Methanolic With Some Antibiotics Against S. Aureus, E-Coli and Klebslia Isolated From the Eye Affected by Inflammation

Abstract

The current study included the collection of (300) samples from (150) people from patients visiting Balad General Hospital and some private clinics in Balad District - Salah Al-Din Governorate, a sample was taken from both the right and left eyes using special dry and sterile cotton swabs that can be disposed of later, for the period ranging from November 2022 to January 2023, (235) positive samples for bacterial growth were obtained, constituting 78.3% of the total samples collected, positive growth samples were diagnosed depending on their morphological, farming and biochemical properties, and the diagnosis was confirmed for a number of them using the Vitek2 system technology. The results showed obtaining 140 isolates out of a total of (235) positive samples for the Gram stain by (59.6%), while the negative isolates of the Gram stain were (95) isolates and amounted to (40.4%) of the total isolates, S. aureus was 27.65% and E-coli 10.63%, while Klebsiella was 8.51% of the total bacterial isolates isolated from the eye, including (staphylococcus aureus, staphylococcus epidermidis, Bacillus, Streptococcus Viridians, Diphtheria, Pseudomonas aeruginosa, Klebsiella, E-coli, Neisseria). The results showed the synergistic effect of the alcoholic extract (methanol) of the aloe vera plant (Aloe Vera L.)  with antibiotics a significant effect compared to methanol alcoholic extract alone, the results of the study also showed that the antibiotic Ciprofloxone synergistically with the alcoholic extract methanolic of the aloe vera plant (Aloe Vera L.)  it had the highest effect already synergistically with most of the bacterial species under study.