Spectrophotometric Determination of Paracetamol Drug by Use Methyl 2- Phenyl Salponyl Acetate (M10) Lap Reagent Prepared

Abstract

Sensitive, fast, accuracy spectrophotometric method developed to determination of paracetamol drug depend on reaction between drug and sodium nitrite solution in acid medium to produced dizonium salt and react with M10 reagent in basic solution, the reaction produced a final yellow and stable product that remained unchanged for over 60 minutes, providing sufficient time to analyze multiple samples. The highest absorption occurred at 432 nm, and the reaction follows Beer's law applies to concentrations between 5 and 40 μg/ml. The molar absorption coefficient is 2262.21 liters/mol/cm, and the Sandel sensitivity is 0.0568 µg/cm^2, with a correlation coefficient (r) of 0.9996. The Limit of Detection (LOD) is 0.2522 µg/ml, and the Limit of Quantification (LOQ) is 0.7641 µg/ml. This method was successfully used to analyze paracetamol in pharmaceutical preparations.