Molecular investigation of Her2 gene in Breast Cancer Women In Babylon City

Abstract

Background: The most prevalent illness in women is breast cancer, “Human epidermal growth factor receptor 2 (HER2)” plays a significant role in the carcinogenesis of breast cancer. (HER2)” overexpression can arise from several causes and is frequently observed in 20–30% of instances of breast cancer.

Aim of the study: The current study was aimed to investigate the correlation between the existence of HER2 gene expression in the peripheral blood samples with breast cancer females by using the primers of HER2 gene and PCR technique.

Materials and Methods: In this study, seventy two (72) peripheral Blood samples were taken from the breast cancer ladies from Marjan Hospital's at Oncology Center in Babylon city's who had previously received a confirmatory diagnosis of breast cancer with ten (10) blood samples from clinically healthy women as a control group during the period from June 2022 to January 2023, DNA samples were isolated from the collected blood samples by DNA Extraction kit (FavorPrepTM Blood/Cultured Cells Genomic DNA Extraction Mini Kit), then, conventional PCR technique was performed with specific oligo primers to amplify the Her2 gene's target sequence in those DNA samples, then, agarose gel electrophoresis was applied for detecting the target sequence in the DNA products with the band size 150bp.

Results: 58 / 72(80.5 %) of the DNA samples were positive and 14/ 72 (19. 5%) were negative for Her2 gene in the peripheral blood samples of the breast cancer women.

Conclusions: in conclusion the current study's results demonstrated that 58/ 72 (80.5%) of the peripheral blood samples from breast cancer women were positive and the remaining 14/ 72(19.5%) of the peripheral blood samples were negative for Her-2 gene, “HER2 gene” can be considered as a useful biomarker and an obvious indicator for breast cancer which could be employed for early and confirmatory diagnosis of breast cancer in women.