Isolation and Study of The Effect of Natural Products Isolated from Cordia Myxa Plant on The Level of Lysyl Oxidase Like -2 and Some Biochemical Variables in Mice with Bladder Cancer

Abstract

Using HPLC technology, I separated and diagnosed various natural products from the Cordia myxa plant, identifying the oily substance and its fatty acids. Some flavonoids and alkaloids were also separated. We identified the flavonoids using high-performance liquid chromatography (HPLC) technology. The effect of these products on lysyl oxidase like -2 (LOXL-2) was studied, and it was found that they had a positive effect on reducing the level of LOXL-2 and some other measured variables. In addition, the study examined the effect of these natural products on some biochemical parameters related to bladder cancer in the blood serum of mice induced with the disease. Biochemical variables included LOXL-2, glutathione peroxidase, glutathione, malondialdehyde (MDA), and tumor necrosis alpha (TNF-alpha). The results showed that the concentrations of LOXL-2, MDA, and TNF-α increased significantly in infected mice compared to healthy mice, and a significant decrease in the concentration of GPX and GSH was observed. Natural products demonstrated a positive therapeutic effect in mice, and tissue sections of experimental animals established the disease.