Study the Role Of Mirna -126 Gene Expression In A Sample Of Breast Cancer Iraqi Women

Abstract

Background: MicroRNAs play a major function in gene expression and are associated with numerous cancer types. Breast cancer (BC) is aberrant breast cell proliferation induced by environmental and genetic factors with gene expression alterations. Objective: Study of clinical characteristics of women with breast cancer and estimation of circulating miR-126 expression in serum. Methods: This research involved the recruitment of a cohort consisting of seventy female BC patients from Oncology Unit of Al-Yarmouk Teaching Hospital located in Baghdad city, Iraq. Furthermore, thirty samples of healthy female volunteers were included as control subjects. The miR-126 expression levels were measured by means of a technique called real-time reverse transcription-polymerase chain reaction. Results: The age range between 45 to 54 included the higher number of patients with breast cancer comprised treated and untreated groups than other. Roughly 75% of the patients were discovered at an early stage (II), and all patients were in stages II and III. The majority of patients were found to be overweight or obese. This assignment also demonstrated that treated (60.0%) and untreated (68.6%) left breasts had more malignancies than right breasts. Active smokers were rare, whereas non-smokers and passive smokers dominated the group. This study found a substantial drop in miR-126-5p gene expression in BC patients' serum (treated and untreated) compared to healthy controls. Conclusion: The study found a decrease in circulating miR-126-5p gene expression in BC patients' serum.