Chemical Profiling of Kalangkala (Litsea angulata) Seed Using LC-HRMS as Antioxidants

Abstract

Kalangkala (Litsea angulata) is one of the most frequent florae utilized in traditional medicine in the Kalimantan area, and this showed significant antioxidant activity on the part of the seeds. Ability antioxidants it has seed sometimes become potency development material natural as material standard medicine, so need did its identification compounds present in seeds sometimes. Research purposes: this is to identify compound potential antioxidants as candidate materials for active pharmacy. The method used is to test the activity of antioxidants using the DPPH technique from the n-hexane, ethyl acetate, and methanol kalangkala seed fractions, achieved by identifying components using LC-HRMS. Research results show that the IC50 values from the n-hexane, ethyl acetate, and methanol kalangkala seed fraction are, respectively, 52.05, 46.10, and 32.84 ppm. Identification results compound from methanol fraction with activity antioxidant best show 5 major compounds with abundant more than 3% are 3-[(2E)-3,7-Dimethyl-2,6-octadien-1-yl]-4-hydroxy-5-methoxybenzoic acid, 2-[(2E)-3,7-Dimethyl-2,6-octadien-1-yl]-5-hydroxy-6-methoxy-3-methyl-1,4-benzoquinone, Betaine, Choline, and (4E,6E)-4,6-Tetradecadiene-8,10,12-triyn-1-yl acetate.