The Toxicity Assessment of The Nanohydroxyapatite, Epigalocathecine-3-Gallate, And Hydroxyprophil Methylcellulose Hydrogel

Abstract

The previous study showed that the hydroxyapatite, Epigalocathecine-3-Gallate (EGCG), and hydroxypropyl methylcellulose (HAp-EGCG-HPMC) material has the potential to be combined into a useful material in the field of dentistry, and one of them is as a pulp regeneration material. To determine the safety of the HAp-EGCG-HPMC formulation, the characterization of this new material should be performed using the cytotoxicity and proliferation test. It can show the number of living cells and it can indicate the vitality mechanism in certain proteins, also knowing the cell survival after exposure to HAp-EGCG-HPMC material. The method used to detect cell proliferation is commonly similar to the viability test. The cytotoxicity and proliferation were performed Methods: The HAp-EGCG-HPMC hydrogel was made by dissolving hydroxyapatite powder in distilled water to make a concentration of 1%, 2%, 4% with 10μmol/mL EGCG, and hydroxypropyl methylcellulose carriers were added. The cytotoxicity and proliferation in fibroblast cells of HA-EGCG-HPMC hydrogel was done using by CCK-8 dye and read by spectrophotometer with 450nm wavelength. Results: Hydroxyapatite, EGCG, and HPMC hydrogels were nontoxic to fibroblast cells 24 hours after mixing and it can induce proliferation of fibroblast cells. Conclusion: The hydrogel containing hydroxyapatite, EGCG, and HPMC is non-toxic and promotes the proliferation of fibroblast cells, making it suitable as a material for pulp regeneration.