Distribution of TEM and SHV Genes in ESBL-Producing Klebsiella pneumoniae Strains Isolated From Various Clinical Samples in Al-Basrah Province, Iraq

Abstract

Klebsiella pneumonia is an opportunistic pathogen causes several diseases including sepsis, pneumonia, and wound infections. Antimicrobial resistance (AMR) in MDR K.pneumonia carrying genes for extended-spectrum β-lactams (ESBLs) has been considered a risk for health problems around the world, which has greatly limited treatment options. The current study relied on n=26 Klebsiella pneumoniae isolates that were previously isolated by the studies of master’s student “Intithar Mahdi Mozan" between October 2022 and  December 2022,the samples were collected from three hospitals in Al-Basrah province,Iraq. K. pneumoniae isolates reactivated and re-identified by cultivating isolates on MacConkey agar and Klebsiella pneumoniae chromogenic media and then re-identified K. pneumoniae isolates through diagnostic gene 16S rDNA by PCR technique, the results revealed that all n= 26 isolates of K. pneumoniae had a molecular weight of approximately  130 bp at a 100%. Double disk approximation test (DAM) was used to detected produced extended-spectrum β-lactams (ESBLs), the result of current study was showed positive results for the ESBLs. Extended-spectrum β-lactamses (ESBLs) genes was detected by using three specific primers: blaTEM, blaSHV and, blaCTX-M. Out of  n=26 K. pneumoniae isolates results was shown the 23 (88.46%) K. pneumoniae isolates had gave positive results for the blaTEM gene. While 3 (11.538%) K. pneumoniae isolates revealed negative results for blaTEM gene. On other hand results were shown that only 5 (19.23%) K. pneumoniae isolates had positive results for blaSHV gene and 21 (80.769%) K. pneumoniae isolates had negative results for the blaSHV gene. Also the results shown the 5(19.23%)K. pneumoniae isolates had positive results for blaTEM and blaSHV genes.