Investigating Ceremagnum Plus as a Potential Bioceramic Substitute for Pulp Capping Agent: Evidence from Zebrafish Larvae Studies

Abstract

Objectives: Endodontic therapy focuses on preserving or restoring the function and vitality of pulp and periapical tissues. The introduction of calcium silicate-based bioceramics, known for their excellent biological properties, has significantly improved the success rates of endodontic treatments.This study aimed to assess toxicity of indigenously developed ceremagnum plus as a pulp capping agent by investigating its effects on zebrafish larvae survival rate,heart rate and hatching rate by comparing it to calcium silicate based cement MTA.
Materials and Methods: Zebrafish larvae were exposed to different concentrations of Ceremagnum plus and Mineral trioxide aggregate (MTA). Developmental toxicity was assessed by measuring heart rate, survival rate, and hatching rate through microscopy. Statistical analysis was performed using GraphPad Prism software (version 5.03, GraphPad Software, LLC, San Diego, California, USA). A one-way ANOVA followed by Tukey’s post-hoc test was conducted to determine significance levels (p < 0.05) among the control and experimental groups, based on triplicate means and standard deviation.
Results: Ceremagnum plus showed 100% survival rate at all concentrations (1mg/ml,5mg/ml and 10mg/ml) whereas MTA showed decreased rate of 95% at 10mg/ml conc.In this study,Ceremagnum plus didn’t yield a significant change in heart rate at various concentrations (1mg/ml,5mg/ml and 10mg/ml) as compared to decreased rate shown by control group.No significant differences were seen when compared to control group at various concentrations for hatching rate.
Conclusion: Utilising zebra fish experiment ,ceremagnum plus demonstrated favourable attributes, notably enhancing the survival rates of larvae with minimal alterations in heart rates and hatching rates as compared to commercially available MTA, presenting a promising avenue for enhanced biocompatibility.