Comparative Analysis of Methanol and Aqueous for Antioxidant Potential of Citrus Limon Leaf Extracts from Puliyankudi, India

Abstract

Plants constitute an important source of active natural products which differ widely in terms of structure and biological properties. Among these, the leaves of various plants, including citrus species, are particularly noteworthy due to their rich composition of bioactive compounds. When processed into powder, Citrus limon leaves offer a versatile form that can be easily incorporated into various applications, enhancing both their utility and efficacy. This study investigates the antioxidant activity of edible Citrus limon leaves powder collected from Puliyankudi, known as the "lemon city of India," situated in the Tenkasi district, Kadayanallur taluk, Tamil Nadu. The mineral composition of the botanical product was analyzed using methods including X-ray diffraction (XRD) and found out the qualitative and quantitative phytochemical analysis of citrus limon leaf powder by using to extract methanol and aqueous respectively. The antioxidant activity of Citrus limon leaves powder was assessed through a comprehensive range of assays, including DPPH, ABTS, H2O2, FRAP, and Total Antioxidant Activity, using both Aqueous and Methanol extracts. The results demonstrated significant antioxidant efficacy, showing high performance across all tested parameters. These findings highlight the potential of edible Citrus limon leaves powder as a valuable source of antioxidants, suitable for various applications in functional foods and nutraceuticals.