THE EFFECT OF ENDOMETRIOSIS ON MALONDIALDEHIDA (MDA) AND 8-HYDROXY-2-DEOXYGUANOSINE (8-OHdG) IN OOCYTE OF ENDOMETRIOSIS MODEL MICE AFTER OVULATION

Abstract

The mechanism of endometriosis causing infertility has not yet been fully explained. Lipid peroxidation is one of the pathways that causes a decrease in oocyte quality due to the influence of endometriosis, resulting in infertility. Malondialdehyde (MDA), which is a product of lipid peroxidation, will affect the increase in 8-hydroxy-2-deoxyguanosine (8-OHdG) so that the DNA damage occurs. The purpose of this study is to prove the effect of endometriosis on the levels of MDA and 8-OHdG in oocytes of endometriosis model mice.This study used 32 mice which are divided into endometriosis model mice (P0) and healthy mice (P1) as controls. Endometriosis model mice were made by injecting intraperitoneally the endometrial tissue from patients diagnosed with endometriosis. Oocytes obtained from each mouse were separated from the Oocyte-Cumulus Complex (OCC) then divided into two and each was examined for MDA and 8-OHdG levels by ELISA.It was found that MDA and 8-OHdG levels were significantly different in endometriosis model mice compared to healthy mice (p<0.05). Pearson correlation test between MDA levels and 8-OHdG levels in endometriosis model mice showed no significant relationship (p>0.05).MDA and 8-OHdG levels were higher in endometriosis model mice compared to healthy mice. There was no relationship between increased levels of Malondialdehyde (MDA) and increased levels of 8-hydroxy-2-deoxyguanosine (8-OHdG) in endometriosis model mice.