Detected gene hymolysin in staphylococcus aerues

Abstract

  Staphylococcus aureus is a significant human pathogen characterized by diverse virulence factors that contribute to its pathogenicity. this organism remains a leading cause of  both hospital –acquired and community –associated infections worldwide the hemolysin gene plays an important role in bacterial virulence through its cytolytic activity against host cells

Material and methods: Thirty clinical samples were collected and directly inoculated onto blood agar and mannitol salt agar, followed by incubation at37 ºC for 18-24 hours.  Bacterial identification was confirmed through standard microbiologyical techniques including gram staining, catalase test, and coagulase test. DNA extraction PCR amplification of the hemolysin gene was performed to detect the hemolysin gene was conducted using specific primer. The amplified products were sequenced and analyzed for genetic characterization then phylogenetic tree for genes sequenced was constructed by using (MEGA10).                               

 Results: PCR analysis succefully amplified the hemolysin gene for the clinical isolates.  eight PCR products sequenced and submitted to GeneBank receving accession numbers LC78668871 LC78668873,     LC78668874 LC78668875, LC78668876, LC78668877 LC78668878, LC78668879, LC78668880.  were recorded in the database. The prevalence of the hemolysin gene among the tested isolated was 100%   indicating its  significance in the virulence profile of local S. aureus strains of the through PCR amplification provides a potential rapid ndiagnostic tool for identifying S. aureus infections directly from clinical specimens. The presence of this gene indicates the prevalence of highly virulent and pathogenic strains of S. aureus. Specimens                                                     

Conclusion: The PCR for amplification of HLY gene has potential for rapid diagnosis of S. aureus infections by direct testing of clinical specimens. The Sequence gene  is  one  of  the  modern  advanced development  technique  in  molecular  biology.  In this  way  genetic  relationship  can be  detected  between bacterial isolates rapidly