ANTI-INFLAMMATORY ACTIVITY OF SEED EXTRACT OF CARICA PAPAYA : AN IN-VITRO STUDY

Abstract

The most frequently used non-steroidal anti-inflammatory drugs (NSAIDs) to treat inflammatory conditions only alter the inflammatory response to the diseases, not the underlying cause of the disease. Moreover, long-term use of NSAIDs leads to serious side effects. Hence, the current study was designed with the main purpose to evaluate the anti-inflammatory activity of methanolic seed extract of Carica papaya. Seeds of C. papaya was subjected to successive solvent extraction by continuous hot extraction (Soxhlet) with methanol. The in-vitro anti-inflammatory activity of methanolic seed extract of C. papaya was determined using albumin denaturation method. Qualitative analysis revealed major phytochemicals such as alkaloids, saponins, phenolic compounds, and tannins in methanolic seed extract of C. papaya. Quantitative estimation revealed 182.45 mg GAE/g and 24.12 mg GAE/g quantities of total phenolic compounds and tannins in methanolic seed extract of C. papaya. There was dose dependent inhibition (%) was observed in standard as well as methanolic seed extract of C. papaya. Furthermore, the inhibition (%) of methanolic seed extract of C. papaya at the concentration of 500 µg/mL was at par with that of standard drug i.e., Aspirin. In conclusion, methanolic seed extract of C. papaya could be explored in the development natural anti-inflammatory drugs.