NEPHROPROTECTIVE AND DIURETIC ACTIVITY OF C. INDICUM LEAVES EXTRACT ON EXPERIMENTAL ANIMALS

Abstract

Investigating the nephroprotective and diuretic effects of C. indicum ethanol extract at two doses of 250 and 500 mg/kg bw on gentamycin-induced toxicity in rats was the goal of this investigation. Gentamicin (100 mg/kg) was administered to all animal groups to assess nephroprotective effect; acute renal dysfunction is demonstrated by markedly elevated serum creatinine, urea, and uric acid, as well as lower body weight and numerous histological abnormalities. At doses of 250 and 500 mg/kg, treatment with C. indicum has demonstrated a significant (p<0.05, p<0.01 and p<0.001) dose-dependent improvement in body weight. It has also demonstrated a significant improvement in kidney protection against oxidative stress. Additionally, it was found that, in comparison to the toxic group, treatment with C. indicum considerably reduced the levels of serum creatinine, uric acid, and urea. Serum creatinine, total protein, kidney weights, and body weights were among the parameters that showed nephroprotective effect of EECI treatment when compared to the standard group (Vitamin E, 250 mg/kg) and control group animals against the toxic control group. The protective effect of EECI was further demonstrated by the histological investigations. Additionally, two doses of the C. indicum ethanol extract had a substantial diuretic effect by raising levels of both total urine production and urinary electrolytes including potassium and sodium. Ultimately, these findings indicate that C. indicum ethanol extract has nephroprotective properties against gentamycin-induced nephrotoxicity and strong diuretics effect in rats.