Synthesis, physiochemical assessment, characterisation, and anticancer activity of 2-chloro-N-(4-((2,3-dihydro-4H-1,4-oxazin-4-yl)sulfonyl) phenyl) acetamide derivatives

Abstract

Medicinal chemistry focuses on the exploration and advancement of new pharmaceuticals for the treatment of diseases. The primary objective of the field is to create innovative organic molecules, whether they are naturally occurring or artificially synthesised. Heterocyclic nuclei are a substantial part of these chemical molecules. Sulphonamide based acetamide molecules are currently being utilised in the development of novel anticancer medicines. The physicochemical characteristics of the synthesised substance (SA-1 to SA-3) were documented. The chemical SA-1 to SA-3 has been characterised using infrared spectroscopy (IR), proton nuclear magnetic resonance (1HNMR), carbon-13 nuclear magnetic resonance (13CNMR), and mass spectral analysis. Additionally, all the other synthesised compounds have exhibited a molecular ion peak that is analogous to their respective molecular formula and weight. The synthesised compounds were assessed for their anticancer activity against the NCI-H226 lung cancer cell line using the SRB test technique. The MTT experiment indicated that compounds SA-1, SA-2, SA-3 had significant efficacy against the Lung cancer cell line. It demonstrated a significant cytotoxic effect on both cell types, particularly on the WI-38 normal lung fibroblast cells and NCI-H226 lung cancer cells. Evidently, all compounds that were examined demonstrate a notable induction of cell death, except for SA-1, SA-2, SA-3, which had IC50 values of 1866.20, 1702.23, 1374.35 µg/ml, respectively.