"SYNTHESIS AND IN VITRO EVALUATION OF NITROGEN-CONTAINING DERIVATIVES AS POTENTIAL ANTICANCER AGENTS"

Abstract

This study investigates the characterization and cytotoxic effects of various synthesized compounds and 5-Fluorouracil (5-FU) against the MCF-7 breast cancer cell line. Characterization of the compounds revealed yields ranging from 60% to 85%, with significant differences in Rf values, reflecting variations in polarity. IR spectroscopy identified key functional groups including C=C-H, C=C Ar, C=O, C-Cl, and C-Br, with unique peaks indicating the presence of additional groups such as N=O and O-H. NMR spectroscopy further delineated the structural characteristics of the compounds, highlighting their distinct substitution patterns. The MTT assay results demonstrated that 5-FU exhibited the highest percent inhibition at 78.99% with an average absorbance of 0.247, showing the strongest cytotoxic effect. Among the synthesized compounds, 1a, 1b, and 1c displayed percent inhibitions of 37.78%, 43.02%, and 46.79%, respectively, while compounds 2a and 2b showed higher inhibitions of 63.60% and 64.08%, indicating their potential as effective alternatives in breast cancer treatment. The data suggests that while 5-FU remains the most potent, several synthesized compounds exhibit promising anti-cancer activity, warranting further exploration.