COMPARATIVE CYTOTOXIC EFFECT OF COMMERCIAL MOUTHWASH AND GREEN TEA PAPAYA FORMULATION BASED MOUTHWASH - AN IN VITRO STUDY

Abstract

INTRODUCTION : An ideal mouthwash is one that doesn't cause allergies, doesn't discolor teeth or the oral mucosa, has anti-plaque and antibacterial qualities, doesn't have a lot of cytotoxic effects on cells, is environmentally friendly, and tastes good. The aim of the present study is to compare the cytotoxic effect of commercial mouthwash and green tea and papaya formulation based mouthwash.
MATERIALS AND METHODS :
The study was conducted in the month of August 2022 in Saveetha dental college and Hospitals. Papaya and green tea samples were collected and the samples were boiled, filtered and concentrated to prepare the extract. Both the extract were mixed together and 0.3g sucrose, 0.01 g SLS agent and sodium benzoate was added and mouthwash was prepared. Cytotoxic activity was compared with commercial chlorhexidine mouthwash using brine shrimp lethality assay. Salt water was prepared. 6 ELISA plates were taken with different concentrations and 10-12 ml of saline water was filled. To that 10 nauplii were slowly added to each well Then the nanoparticles were added according to the concentration level. The plates were incubated for 24 hours, the ELISA plates were observed and noted for the number of live nauplii present and calculated by using formula. The results were then tabulated and represented graphically
RESULTS : The cytotoxic activity of commercial mouthwash and green tea and papaya formulated mouthwash was compared. The number of live nauplii present in commercial mouthwash and papaya and green tea formulated mouthwash decreased on increasing the concentrations. But compared to commercial mouthwash, green tea and papaya formulated mouthwash had a higher number of nauplii present.
CONCLUSION : From the present study it was concluded that the papaya green tea formulated mouthwash showed lower cytotoxicity than commercial mouthwash.