Assessment of Erymax30 in-vivo LC50 Reference Values in Snake-headed murrel (Channa punctatus Bloch)

Abstract

The in-vivo acute toxicity, median lethal concentration, and safe concentration of Erymax 30 in an Indian snake-headed murrel, Channa punctatus (Bloch), weighing 50-60g, were assessed using various methodologies. Lorke and Enegide et al. estimated the 96-hour LC50 value as 189.7 and 190.0 mg/l, respectively. As an approximate estimate, the Up-and-Down approach produced a 96-hour LC50 range of 200.0 to 210.0 mg/L. Furthermore, the Behren-Karber, Miller, Tainter, and Finney techniques reported 96-hour LC50 values of 222.0, 205.17, and 192.75mg/l, respectively. Finally, the application of the Reed-Muench method calculated the 96-hour LC50 dose and the median optimum dose of 208.45mg/l. In Channa punctatus, the cumulative median lethal average of Erymax 30 at 96 hours was 222.0+205.17+192.75+208.45=207.09 mg/l. The doses of Erymax 30 have a significant relation to fish mortality. According to the data, Erymax 30 accumulates in aquatic bodies to disrupt this fish's physiological activity. As a result, while treating Channa punctatus with high dosages of Erymax 30, proceed with caution.
Channa punctatus is less susceptible to Erymax 30 toxicity than other fish. The computed safety level value revealed that identifying an acceptable dose of Erymax 30 was possible. Erymax 30 could be considered a moderately/highly hazardous drug to Channa punctatus.