Combination of Leucine- Fluoroquinolone in Inhibiting Dental Pathogen Biofilms

Abstract

Purpose: Leucine, an essential amino acid, has shown potential in modulating bacterial growth, with potential applications in dentistry and oral health. When used in conjunction with fluoroquinolones, which are antibiotics known for their broad-spectrum activity, it is effective against a variety of bacterial pathogens. The principal aim of the study was to assess the combinatorial efficacy of leucine - fluoroquinolone antibiotics in the deterring of biofilm formed by prominent dental pathogens.
Materials & Methods: 100 mL of Brain Heart Infusion broth was inoculated with 4-5 colonies of S.mutans and E.faecalis and incubated at 37 degree C overnight in an incubator. Leucine & Leucine - fluoroquinolone was diluted to desired concentrations and added to BHI broth in the wells & was inoculated with 50 mL of broth culture. The Broth was aspirated, washed, & 150 µL of crystal violet was added to each well & allowed to stand for 15-20 minutes. The dye was removed, washed, and then dissolved by adding 150 µL of 30% glacial acetic acid in each well. Reading was taken using ELISA plate reader at 570 nm.
Results: The optical density of leucine was comparatively higher than the combination of leucine and ciprofloxacin in case of both Streptococcus mutans and Enterococcus faecalis, demonstrating that proportion of biofilm formation with S.mutans & E.faecalis had decreased when treated with leucine-ciprofloxacin antibiotic combination than with leucine alone.
Conclusion: Leucine has potential anti-biofilm activity if used as an adjuvant along with an antibiotic against drug resistant strains of bacteria such as S.mutans & E.faecalis.