Functional And Histological Effects Of Organic Selenium On Ovaries Of Female Rats Induced ‎With Hypothyroidisms

Abstract

The present study examined the association between hypothyroidism and reproductive ‎dysfunction by the analysis of hypothyroidism effect on gonadotropin secretion in cyclic ‎female rats, which is rectified by supplementation of these animals with selenium. Forty cyclic ‎females were created to four groups (10 each). The control fed normally. First females were ‎injected with 10 mg/kg propylthiouracil‏ , ‏Hypothyroidism was induced in the second and ‎third groups using PTU, followed by treatment with selenium alone or with zinc at 7 mg and 10 ‎mg/kg body weight, respectively. Blood samples were collected at late estrus to measure levels ‎of thyroxin(T4), triiodothyronine(T3), thyroid–stimulating hormone(TSH), follicle-stimulating ‎hormone(FSH), estradiol, and inhibin. Also, Morphological, histological changes, and ovarian ‎function were examined. The hypothyroid group showed a decrease of T4, T3, FSH, and E2 ‎levels and decreased ovary weight and diameter in parallel with the number of primary, ‎secondary, and Graafian follicles; in the meantime, there were increased levels of TSH and ‎inhibin concentrations. Treatment with selenomethionine alone or with zinc diminished some ‎of the adverse consequences of the depressing action of the thyroid, leading to marked ‎improvement or normalization of the hormone levels together with the results of the ovarian ‎histological structure. It was identified that PTU caused hypothyroidism. In contrast, in the case ‎when female white rats were treated with selenomethionine alone or with zinc, an effect was ‎exerted to reduce the PTU depressive action on the system of female reproduction and the ‎quantity of the sexual hormones.‎