Correlation Of Salivary Malondialdehyde (MDA) And 8-Hydroxy-Deoxyguanosine (8-Ohdg) In Residual Ridge Resorption In Completely Edentulous Diabetic Patients

Abstract

Aim: The aim of this study is to correlate the salivary Malondialdehyde (MDA) and 8-Hydroxy-deoxyguanosine (8-OHdG) in residual ridge resorption in completely edentulous diabetic patients.

Materials and Method:

The study included completely edentulous diabetic and non-diabetic patients, selected based on specific inclusion/exclusion criteria. A total of 48 saliva samples were collected from 24 participants. Saliva was collected under standardized conditions and analyzed for oxidative stress markers—Malondialdehyde (MDA) using spectrophotometry and 8-hydroxydeoxyguanosine (8-OHdG) using ELISA. Residual ridge resorption was assessed radiographically using orthopantomograms and categorized per ADA (1999) criteria. Statistical analyses, including Pearson’s correlation and regression models, were employed to evaluate the relationship between oxidative stress markers and resorption severity. Ethical approval and informed consent were obtained.

Statistical Analysis and Results:

48 saliva samples from diabetic and non-diabetic edentulous patients (n=24 per group). Biomarkers MDA and 8-OHdG were measured using spectrophotometry and ELISA, respectively. Statistical analysis (SPSS v23.0) included parametric and non-parametric tests based on data distribution, with significance set at p<0.05.

Results showed higher mean absorbance levels of MDA and 8-OHdG in non-diabetics compared to diabetics, though differences were not statistically significant. A positive correlation between MDA and 8-OHdG indicated a link between lipid peroxidation and oxidative DNA damage. These findings suggest elevated oxidative stress markers in non-diabetics, potentially reflecting adaptive metabolic responses in diabetics.

Conclusion: within the limitations of the study, Significant role of oxidative stress in residual ridge resorption (RRR), as evidenced by elevated levels of salivary biomarkers MDA and 8-OHdG in edentulous patients. Non-diabetic individuals exhibited higher levels of both biomarkers, suggesting variability in oxidative stress responses, possibly due to adaptive mechanisms in diabetic patients. A strong positive correlation between MDA and 8-OHdG further supports their reliability as salivary indicators for assessing the severity of RRR.