Analytical Method Development And Validation Of Fluticasone Propionate By Using RP- HPLC

Abstract

In this study, a straightforward, reliable, precise, and stable RP-HPLC method for estimating fluticasone propionate. Among the method's many benefits are its straight forward and mobile phase, inexpensive solvents, quick analysis. HPLC system used was JASCO system equipped with model PU 4180 RHPLC pump. rheodyne sample injection port (20 µl), JASCO UV-4075 UV-VIS detector and Chrom NAVCFR chromatography software (version 2.0). Separation was carried out on HiQSil C18 (250 mm x 4.6 mm, 5 µm) column using methanol and water as mobile phase at flow rate of 1.0 min. Samples were injected using Rheodyne injector with 20 µL. loop. Detection was carried out at 235nm, with  a sharp peak at 4.00 minutes for fluticasone propionate work .the method exhibits good linearity (r2 = 0.9999). The % RSD values for method precision and intermediate precision studies were found to be less than 2%. The % recovery was found to be within an acceptable limit 98%-102%.Thus, the created method was described as robust, accurate, exact, and linear. Because the process eliminates the need for costly reagents and also It takes less time.